T&E - Analítica




About Vitamins and Mineral Salts


Vitamins are organic compounds essential for the normal functioning of human and animal metabolism. They function mainly as catalysts for reactions necessary to our body.
    The vitamins are classified according to their solubility: WATER SOLUBLE or water soluble and FAT SOLUBLE or fat soluble.
     Vitamin D can be synthesized by the body through the exposure of the skin to ultraviolet rays that, when penetrating it, stimulate the 7-dihydrocholesterol (provitamin D3) present in the skin, transforming it into the active form, Vitamin D3, which is metabolized by the liver and the kidneys.
     The main vitamins are described in the table below. As only vitamin D can be synthesized, all the others must be obtained through the alimentary ingestion, which makes a correct feeding of the matter of importance.


Vit A


Lipo solúvel

Vit A

Acetato de Retinol

Lipo solúvel

Vit A

Propionato de Retinol

Lipo solúvel

Vit A

Palmitato de Retinol

Lipo solúvel

Vit A

Betacaroteno – precursor do Retinol

Lipo solúvel

Vit B1


Hidro solúvel

Vit B2


Hidro solúvel

Vit B3 (PP)

Niacina ou Nicotinamida

Hidro solúvel

Vi B4


Hidro solúvel

Vit B5

Pantotenato de cálcio

Hidro solúvel

Vit B6


Hidro solúvel

Vit B7


Hidro solúvel

Vit B8

Biotina ou vit H

Hidro solúvel

Vit B9

Ácido fólico

Hidro solúvel

Vit B10

Ácido Para Amino Benzóico (PABA)

Hidro solúvel

Vit B11


Hidro solúvel

Vit B12


Hidro solúvel

Vit B13

Ácido Orótico

Hidro solúvel

Vit D2


Lipo solúvel

Vit D3


Lipo solúvel

Vit E


Lipo solúvel

Vit H


Hidro solúvel

Vit C

Ácido ascórbico

Hidro solúvel

Vit K1

Filoquinona ou fitonadiona


Vit K2

Menaquinona ou farnoquinona

Lipo solúvel

Vit K3

Menadiona (sintética)

Lipo solúvel


     The so-called VITAMIN COMPLEXES, composed of several species of vitamins and usually accompanied by the called mineral salts, such as calcium; copper; chrome; iron; iodine; magnesium; manganese; molybdenum; selenium, zinc, chlorine, fluorine, iodine, phosphorus, sodium, among others. Mineral salts are essential elements for health, having their main origin from the soil. They are not produced by our body, in this way our bodies receives them from the food, especially in the ingestion of vegetables and water (for both the composition can vary in different regions of the earth).

In humans, minerals participate in chemical reactions in the body, and their intake is a very important factor in maintaining health.




The T&E Analytical Center reviews all Vitamins with extensive experience. All vitamins present in poly vitamins and in different matrices are analyzed in the T & E Center. Common and non-common vitamins are analyzed according to the compendial standards or methodologies developed by the T & E Analytical Center or further developed according to the matrix. In the same way apply to mineral salts and other components, such as omega-type fatty acids, sometimes added to the complex


1.   Vitamins, essentially organic compounds


    The analyzes are performed by chromatographic techniques, in this case the Analytical T & E Center has an analytical instrumental park that can meet the great majority of organic compounds. Techniques such as HPLC (liquid phase chromatography) in different detectors, CFG (gas chromatography) in different detectors. UPLC (high pressure liquid chromatography or fast column chromatography). High-performance equipment such as: LC-MSMS couplings; GC-MS. Finally various techniques to be able to offer resolution or directions to your product. Determination of peak purity using DAD.





    Microbiological analyzes for sterile, non-sterile and antineoplastic products

Determination of Content - Development and Validation, among other tests:


·         Coliformes Thermo tolerant;

·         Search of Salmonella;

·         Countable viable cells;

·         Challenge test (challenge test)

·         Endotoxins LAL

·         Fungos

·         Sterility

·         Determination of content (power)

·         Vitamin analysis

·         Testing in lids and containers,

·         Supports restaurant kitchens in contamination,

·         Sampling and analysis of process air and packaging,

·         Process validation analysis,

·         Verification of industrial contamination.


Some vitamins, such as B12 or H (biotin) are classical analyzes by the microbiological technique, but are also analyzed by HPLC and in the case of vitamin H (biotin) by the LC-MSMS coupling.


2.   For the Mineral Salts that in addition to metals include, amethals and semi-metals, the following techniques are available at the T & E Center:


·         ICP (Inductively Coupled Plasma) Sequential and Simultaneous,

·         AA (Atomic Absorption);

·         FC (Flame Photometry);

·         Medical techniques such as: selective electrodes, potentiometry, complexiometry, colorimetry, among


3.   For anion analysis, the following techniques are proposed:


·         Colorimetry, Complexiometry, Gravimetry and others


More techniques not described here, as well as the need for analytical solutions involving new techniques or their arrangement, are available to evaluate and direct directions and how to solve them.